1. Cell structure and Function

1.5.a. Cell organelles of both Plant and animals

Cell organelles of both plant and animal cell

Dr V Malathi and Mrs Sushumna Rao

The Nucleus

The nucleus (plural = nuclei) is the most prominent  and largest organelle in a cell. It is the cell’s control center.

The DNA is present in the nucleus. The DNA is encoded with the genetic information.

Nucleus contains a thick liquid called nucleoplasm

The composition of nucleoplasm is similar to that of cytoplasm.

Most eukaryotic cells contain just a single nucleus.

Some types of cells  like red blood cells contain no nucleus and a few other types of cells  such as muscle cells contain multiple nuclei.

The nucleus is surrounded by a double membranous structure called the nuclear envelope.

The tiny holes are present  in the nuclear envelope and these are called nuclear pores through which molecules move in and out of the nucleus.

The structure called nucleolus is  housed inside the nucleus. It is mainly involved in the assembly of ribosomes.

The ribosomes after being produced in the nucleus are exported to the cytoplasm, where they are involved in the synthesis of proteins.

 

 

In this illustration, chromatin floats in the nucleoplasm. The nucleoid is depicted as a dense, circular region inside the nucleus. The double nuclear membrane is perforated with protein-lined pores

“Nucleus” by CNX OpenStax, is licensed under CC BY 4.0

Watch the video about the Nucleus of the cell

Mitochondria

The mitochondrion ( plural mitochondria) are the “powerhouse of a cell”  as they make energy required for a cell .

They use organic compounds such as glucose and through metabolic process called , and  make ATP ( Adenosine Triphosphate molecules)

Mitochondria contain their own DNA and ribosomes!

 

Mitochondria have a complex structure including an inner and outer membrane.

The inner membrane contains  a version of cytoplasm, called matrix.

 

Can mitochondria be considered a cell?  as they have there own DNA and ribosomes.

To explain this scientists have put forward a theory called Endosymbiotic theory.

According to this theory mitochondria  were once considered to be  free-living organism as they contain their own DNA and were considered as ancient prokaryotic cells.

These cells were then engulfed by larger prokaryotic cells.

The two cells then evolved a symbiotic relationship where the larger cells provided the smaller prokaryotes with a place to live. In return, the larger cells got extra energy from the smaller prokaryotes.

Eventually, the smaller prokaryotes became permanent guests of the larger cells and stayed as organelles inside them.

 

Endoplasmic Reticulum

The endoplasmic reticulum (ER) is a series of interconnected membranous tubules.

There are two separate areas of the endoplasmic reticulum: the rough endoplasmic reticulum and the smooth endoplasmic reticulum, respectively.

The hollow portion of the ER tubules is called the lumen or cisternal space.

The membrane of the ER is a phospholipid bilayer embedded with proteins and is continuous with the nuclear envelope.

The rough endoplasmic reticulum (RER) :  This is so named because when viewed through the electron microscope ,the ribosomes attached to its cytoplasmic surface give it a studded appearance . The ribosomes synthesize proteins while attached to the ER and  transfer of the  newly synthesized proteins into the lumen of the RER . Here the proteins  undergo modifications such as folding or addition of sugars. The RER also makes phospholipids for cell membranes

The phospholipids or modified proteins that are not destined to stay in the RER, will be packaged within vesicles and transported from the RER by vesicles budding from the membrane. Since the RER is abundant in cells that secrete proteins, such as the liver.

File:Endoplasmic reticulum 4 -- Smart-Servier.png

“Rough endoplasmic reticulum” by Laboratoires Servier, is licensed under CC BY 4.0

The smooth endoplasmic reticulum (SER)  : This is continuous with the RER but has few or no ribosomes on its cytoplasmic surface.It serves the following functions such as :

  •  Synthesis of carbohydrates, lipids (including phospholipids), and steroid hormones;
  • Detoxification of medications and poisons;
  • Alcohol metabolism; and
  • Storage of calcium ions.

File:Endoplasmic reticulum 6 -- Smart-Servier.png

“Smooth endoplasmic reticulum” by Laboratoires Servier, is licensed under CC BY 4.0

The Golgi Apparatus

These are the sorting organelles of the cell .

The golgi apparatus also known as the golgi bodies are a series of flattened membranous sacs.

Before reaching their final destination, the lipids or proteins within the transport vesicles need to be sorted, packaged, and tagged so that they are delivered to the required destination of the cell.

In this transmission electron micrograph, the Golgi apparatus appears as a stack of membranes surrounded by unnamed organelles.This figure shows the nucleus, rough ER, Golgi apparatus, vesicles, and plasma membrane. The right side of the rough ER is shown with an integral membrane protein embedded in it. The part of the protein facing the inside of the ER has a carbohydrate attached to it. The protein is shown leaving the ER in a vesicle that fuses with the cis face of the Golgi apparatus. The Golgi apparatus consists of several layers of membranes, called cisternae. As the protein passes through the cisternae, it is further modified by the addition of more carbohydrates. Eventually, it leaves the trans face of the Golgi in a vesicle. The vesicle fuses with the cell membrane so that the carbohydrate that was on the inside of the vesicle faces the outside of the membrane. At the same time, the contents of the vesicle are released from the cell.

“Golgi apparatus” by Openstax is licensed under CC BY 4.0

   

The Golgi apparatus has a receiving face near the endoplasmic reticulum called the Cis golgi and a releasing face on the side away from the ER called the Trans golgi, toward the cell membrane.

The transport vesicles from the ER travel to the Cis  face, fuse with it, and empty their contents into the lumen of the Golgi apparatus.

As the proteins and lipids travel through the Golgi, they undergo further modifications.

The most frequent modification is the addition of short chains of sugar molecules. The newly modified proteins and lipids are then tagged with small molecular groups to enable them to be routed to their proper destinations.

Finally, the modified and tagged proteins are packaged into vesicles that bud from the Trans face of the Golgi.

Some of these vesicles, transport vesicles, deposit their contents into other parts of the cell where they will be used,

While other secretory vesicles, fuse with the plasma membrane and release their contents outside the cell.

Salivary gland  cells , immune cells that engage in a great deal of secretory activity such as secreting digestive enzymes or  secretion of  antibodies have an abundant number of Golgi.

In plant cells, the Golgi has an additional role of synthesizing polysaccharides, some of which are incorporated into the cell wall and some of which are used in other parts of the cell.

Ribosomes

Ribosomes are the cellular structures responsible for protein synthesis.

When viewed through an electron microscope, ribosomes appear either as clusters (polyribosomes) or single, tiny dots that float freely in the cytoplasm.

They may be attached to the cytoplasmic side of the plasma membrane or the cytoplasmic side of the endoplasmic reticulum and the outer membrane of the nuclear envelope
Ribosomes, which are large complexes of protein and RNA,

They consist of two subunits, called large and small subunit.

Ribosomes receive their “instructions” for protein synthesis from the nucleus,

The DNA in the nucleus is transcribed into messenger RNA (mRNA). This process is called

The mRNA travels to the ribosomes, which translate the code provided by the sequence of the nitrogenous bases in the mRNA into a specific order of amino acids in a protein. This process is called

Figure_04_03_06.jpg

“Ribosomes” by Openstax is licensed under CC BY 4.0

Peroxisomes

These are small, round organelles enclosed by single membranes.

Their chief function are :

  • They carry out oxidation of  fatty acids and amino acids.
  • They also detoxify many poisons that may enter the body.
  • Alcohol is detoxified by peroxisomes in liver cells.

A byproduct of these oxidation reactions is hydrogen peroxide, H2O2, which is contained within the peroxisomes to prevent the chemical from causing damage to cellular components outside of the organelle.

Hydrogen peroxide is then safely broken down by peroxisomal enzymes into water and oxygen.

This diagram shows a peroxisome, which is a vesicular structure with a lipid bilayer on the outside and a crystalline core on the inside.

“Peroxisomes” by Openstax is licensed under CC BY 4.0

Check your understanding about cell organelles

For further reading and practice visit  the Interactive Book on Animal and plant Cell Organelles below

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1.5.a. Cell organelles of both Plant and animals by Dr V Malathi and Mrs Sushumna Rao is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License, except where otherwise noted.

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