{"id":366,"date":"2023-03-16T15:49:44","date_gmt":"2023-03-16T15:49:44","guid":{"rendered":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/?post_type=chapter&p=366"},"modified":"2023-04-07T11:25:19","modified_gmt":"2023-04-07T11:25:19","slug":"eukaryotic-transcription-elongation-termination","status":"publish","type":"chapter","link":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/chapter\/eukaryotic-transcription-elongation-termination\/","title":{"raw":"Eukaryotic Transcription- Elongation & Termination","rendered":"Eukaryotic Transcription- Elongation & Termination"},"content":{"raw":"

The promoters of\u00a0 <\/span>RNA polymerase I<\/span><\/h1>\r\n

The promoters recognized by\u00a0<\/span>RNA polymerase I<\/span> consists of<\/span><\/p>\r\n\r\n

    \r\n \t
  • \u00a0a core element\u00a0<\/strong> :\u00a0 It\u00a0 surrounds the transcription start site , and<\/span><\/li>\r\n \t
  • an upstream promoter element\u00a0 :\u00a0<\/strong> This\u00a0 is about 100 bp farther upstream. <\/span><\/li>\r\n \t
  • RNA polymerase\u00a0 binds to promoter containing a core promoter element and an upstream control element (UCE). The Tata Binding Protein ( TBP), which is part of a larger complex called SL1, helps RNA polymerase I to recognize the core promoter<\/span><\/li>\r\n<\/ul>\r\n

    The promoters of <\/span>RNA polymerase III<\/span><\/h1>\r\n
      \r\n \t
    • The RNA polymerase III promoters are of two types namely\u00a0 Type I and Type II . These\u00a0 promoters\u00a0 lie wholly within the genes.\u00a0<\/span><\/li>\r\n \t
    • The type II promoter of the <\/span>Xenopus laevis<\/em>\u00a05S RNA gene consists of an internal control region (ICR). <\/strong>This is \u00a0subdivided into A box (+50 to +60), intermediate element (IE, +67 to +72), and C box (+80 to +90). <\/span><\/li>\r\n \t
    • The type II\u00a0 promoter of the <\/span>X. laevis<\/em>\u00a0tRNA<\/span>Leu<\/sup>gene consists of an A box (+8 to +19) and a B box (+52 to +62). <\/span><\/li>\r\n \t
    • The type III promoter of the <\/span>Homo sapiens<\/em> U6 snRNA gene consists of a distal sequence element (DSE, \u2212215 to \u2212240)\u00a0 and a core promoter composed of a proximal sequence element (PSE, \u221265 to \u221248) and a TATA box (\u221232 to \u221225).\u00a0<\/span><\/li>\r\n<\/ul>\r\n

      Elongation<\/h1>\r\n
        \r\n \t
      • \r\n

        RNA Polymerase\u00a0 clears or \u201cescapes\u201d the promoter region and leaves most of the transcription initiation proteins behind.<\/p>\r\n<\/li>\r\n \t

      • \r\n

        RNA Polymerases travel along the template DNA strand in the 3\u2032 to 5\u2032 direction .The synthesis of new RNA strands takes place in the 5\u2032 to 3\u2032 direction. i.e.,\u00a0 new nucleotides are added to the 3\u2032 end of the growing RNA strand.<\/p>\r\n<\/li>\r\n \t

      • RNA Polymerases unwind the double stranded DNA ahead of them and rewinds the unwound DNA behind them.<\/span><\/li>\r\n \t
      • RNA strand synthesis occurs in a transcription bubble of about 25 unwound DNA base pairs. <\/span><\/li>\r\n \t
      • About 8 nucleotides of newly-synthesized RNA remain base paired to the template DNA. The rest of the RNA molecules falls off the template\u00a0 and this\u00a0 allows the DNA behind it to rewind.<\/span><\/li>\r\n<\/ul>\r\n

        Termination<\/h2>\r\n
          \r\n \t
        • The termination of transcription is different for the three different eukaryotic RNA polymerases.<\/li>\r\n \t
        • RNA Polymerase I contain a specific sequence of base pairs -11 bp long in humans\u00a0 :\u00a0 18 bp in mice .\u00a0 This sequence is recognized by a termination protein called TTF-1 (Transcription Termination Factor for RNA Polymerase I.).\u00a0<\/strong> This protein binds the DNA at its recognition sequence and blocks further transcription, causing the RNA Polymerase I to detach from the template DNA strand and\u00a0 releases the newly-synthesized RNA.<\/span><\/li>\r\n \t
        • RNA Polymerse II lack any specific signals or sequences that direct RNA Polymerase II to terminate at specific locations. <\/span><\/span>The transcript is cleaved at an internal site before RNA Polymerase II finishes transcribing. The cleavage site occurs between an upstream AAUAAA sequence and a downstream GU-rich sequence separated by about 40-60 nucleotides in the emerging RNA.<\/span><\/li>\r\n \t
        • \u00a0The upstream portion of the transcript is released .<\/li>\r\n \t
        • The remainder of the transcript is digested by a 5\u2032-exonuclease (called Xrn2 in humans) while it is still being transcribed by the RNA Polymerase II. When the 5\u2032-exonulease \u201ccatches up\u201d to RNA Polymerase II by digesting away all the overhanging RNA, it\u00a0 disengage the polymerase from its DNA template strand, finally terminating transcription.<\/span><\/li>\r\n \t
        • A protein called CPSF in humans binds the AAUAAA sequence and a protein called CstF in humans binds the GU-rich sequence. CPSF cleaves the nascent pre-mRNA at a site 10-30 nucleotides downstream from the AAUAAA site. The Poly(A) Polymerase enzyme\u00a0 catalyze the addition of a 3\u2032 poly-A tail on the pre-mRNA .<\/span><\/li>\r\n \t
        • The RNAs transcribed by RNA Polymerase III have a short stretch of four to seven U\u2019s at their 3\u2032 end. This somehow triggers RNA Polymerase III to both release the nascent RNA and disengage from the template DNA strand.<\/span><\/li>\r\n<\/ul>","rendered":"

          The promoters of\u00a0 <\/span>RNA polymerase I<\/span><\/h1>\n

          The promoters recognized by\u00a0<\/span>RNA polymerase I<\/span> consists of<\/span><\/p>\n

            \n
          • \u00a0a core element\u00a0<\/strong> :\u00a0 It\u00a0 surrounds the transcription start site , and<\/span><\/li>\n
          • an upstream promoter element\u00a0 :\u00a0<\/strong> This\u00a0 is about 100 bp farther upstream. <\/span><\/li>\n
          • RNA polymerase\u00a0 binds to promoter containing a core promoter element and an upstream control element (UCE). The Tata Binding Protein ( TBP), which is part of a larger complex called SL1, helps RNA polymerase I to recognize the core promoter<\/span><\/li>\n<\/ul>\n

            The promoters of <\/span>RNA polymerase III<\/span><\/h1>\n
              \n
            • The RNA polymerase III promoters are of two types namely\u00a0 Type I and Type II . These\u00a0 promoters\u00a0 lie wholly within the genes.\u00a0<\/span><\/li>\n
            • The type II promoter of the <\/span>Xenopus laevis<\/em>\u00a05S RNA gene consists of an internal control region (ICR). <\/strong>This is \u00a0subdivided into A box (+50 to +60), intermediate element (IE, +67 to +72), and C box (+80 to +90). <\/span><\/li>\n
            • The type II\u00a0 promoter of the <\/span>X. laevis<\/em>\u00a0tRNA<\/span>Leu<\/sup>gene consists of an A box (+8 to +19) and a B box (+52 to +62). <\/span><\/li>\n
            • The type III promoter of the <\/span>Homo sapiens<\/em> U6 snRNA gene consists of a distal sequence element (DSE, \u2212215 to \u2212240)\u00a0 and a core promoter composed of a proximal sequence element (PSE, \u221265 to \u221248) and a TATA box (\u221232 to \u221225).\u00a0<\/span><\/li>\n<\/ul>\n

              Elongation<\/h1>\n
                \n
              • \n

                RNA Polymerase\u00a0 clears or \u201cescapes\u201d the promoter region and leaves most of the transcription initiation proteins behind.<\/p>\n<\/li>\n

              • \n

                RNA Polymerases travel along the template DNA strand in the 3\u2032 to 5\u2032 direction .The synthesis of new RNA strands takes place in the 5\u2032 to 3\u2032 direction. i.e.,\u00a0 new nucleotides are added to the 3\u2032 end of the growing RNA strand.<\/p>\n<\/li>\n

              • RNA Polymerases unwind the double stranded DNA ahead of them and rewinds the unwound DNA behind them.<\/span><\/li>\n
              • RNA strand synthesis occurs in a transcription bubble of about 25 unwound DNA base pairs. <\/span><\/li>\n
              • About 8 nucleotides of newly-synthesized RNA remain base paired to the template DNA. The rest of the RNA molecules falls off the template\u00a0 and this\u00a0 allows the DNA behind it to rewind.<\/span><\/li>\n<\/ul>\n

                Termination<\/h2>\n
                  \n
                • The termination of transcription is different for the three different eukaryotic RNA polymerases.<\/li>\n
                • RNA Polymerase I contain a specific sequence of base pairs -11 bp long in humans\u00a0 :\u00a0 18 bp in mice .\u00a0 This sequence is recognized by a termination protein called TTF-1 (Transcription Termination Factor for RNA Polymerase I.).\u00a0<\/strong> This protein binds the DNA at its recognition sequence and blocks further transcription, causing the RNA Polymerase I to detach from the template DNA strand and\u00a0 releases the newly-synthesized RNA.<\/span><\/li>\n
                • RNA Polymerse II lack any specific signals or sequences that direct RNA Polymerase II to terminate at specific locations. <\/span><\/span>The transcript is cleaved at an internal site before RNA Polymerase II finishes transcribing. The cleavage site occurs between an upstream AAUAAA sequence and a downstream GU-rich sequence separated by about 40-60 nucleotides in the emerging RNA.<\/span><\/li>\n
                • \u00a0The upstream portion of the transcript is released .<\/li>\n
                • The remainder of the transcript is digested by a 5\u2032-exonuclease (called Xrn2 in humans) while it is still being transcribed by the RNA Polymerase II. When the 5\u2032-exonulease \u201ccatches up\u201d to RNA Polymerase II by digesting away all the overhanging RNA, it\u00a0 disengage the polymerase from its DNA template strand, finally terminating transcription.<\/span><\/li>\n
                • A protein called CPSF in humans binds the AAUAAA sequence and a protein called CstF in humans binds the GU-rich sequence. CPSF cleaves the nascent pre-mRNA at a site 10-30 nucleotides downstream from the AAUAAA site. The Poly(A) Polymerase enzyme\u00a0 catalyze the addition of a 3\u2032 poly-A tail on the pre-mRNA .<\/span><\/li>\n
                • The RNAs transcribed by RNA Polymerase III have a short stretch of four to seven U\u2019s at their 3\u2032 end. This somehow triggers RNA Polymerase III to both release the nascent RNA and disengage from the template DNA strand.<\/span><\/li>\n<\/ul>\n","protected":false},"author":5,"menu_order":16,"template":"","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"pb_show_title":"on","pb_short_title":"Eukaryotic Transcription- Elongation & Termination","pb_subtitle":"Eukaryotic Transcription- Elongation & Termination","pb_authors":["dr-v-malathi"],"pb_section_license":"cc-by-sa"},"chapter-type":[],"contributor":[61],"license":[54],"class_list":["post-366","chapter","type-chapter","status-publish","hentry","contributor-dr-v-malathi","license-cc-by-sa"],"aioseo_notices":[],"part":3,"_links":{"self":[{"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/chapters\/366","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/chapters"}],"about":[{"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/wp\/v2\/types\/chapter"}],"author":[{"embeddable":true,"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/wp\/v2\/users\/5"}],"version-history":[{"count":27,"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/chapters\/366\/revisions"}],"predecessor-version":[{"id":1061,"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/chapters\/366\/revisions\/1061"}],"part":[{"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/parts\/3"}],"metadata":[{"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/chapters\/366\/metadata\/"}],"wp:attachment":[{"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/wp\/v2\/media?parent=366"}],"wp:term":[{"taxonomy":"chapter-type","embeddable":true,"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/pressbooks\/v2\/chapter-type?post=366"},{"taxonomy":"contributor","embeddable":true,"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/wp\/v2\/contributor?post=366"},{"taxonomy":"license","embeddable":true,"href":"https:\/\/pressbooks.justwrite.in\/understanding-gene-regulation\/wp-json\/wp\/v2\/license?post=366"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}